Inputs & Outputs

Inputs & Outputs: LCIA

As discussed in section 3.1, biomaterials being such a nascent field means there are very few published LCAs at this time. When trying to understand the potential impacts for a particular bioinnovation it helps to start with a highly simplified version of stage 2 of a typical LCA process: the life cycle inventory analysis (LCIA).

Identifying the key inputs and outputs of a biomaterial process enables brands to start to form a comparative, high level risk analysis - for example, might this process be water or energy intensive?, how is the innovator thinking about mitigating this down the line? It may also enable the beginnings of a process to work with an innovator to track and report data as the technology evolves. 

It should be noted however, that innovators may vary on how much they are willing to share while still in development for the reasons stated in 3.1.

ISO 14040

Overarching standard encompassing all LCA phases

Areas of impact

In the absence of a more comprehensive study, the first step is to review areas of potential impact in broad strokes. Below we have included some of the key high level questions that are relevant to all new bioinnovations in terms of impact.

Feedstock

A feedstock is defined by the Oxford dictionary as a “raw material to supply or fuel a machine or industrial process.” 

For bioinnovations the word feedstock can refer to multiple things - from what is ‘fed’ into the process (e.g. biomass), through to what is the nutrition for an organism. Even the organism itself could be viewed as a feedstock as it goes into the process. Because all of these innovations deal with biology in some form the key areas of impact will likely be twofold: 

1) Is the feedstock genetically modified in anyway, and is any of the GMO material left in the end product.

2) If the feedstock is a crop, where is it produced, is it produced sustainably and could the land used be used to grow food or feed instead?

Energy & Emissions

As with any manufacturing processes, there will be energy implications for running equipment and plants. This is no different for bioinnovations. So it’s important to track energy consumption and identify early on any particularly energy intensive parts of the process. 

For emissions, these are of course closely tied up with energy consumption. And as for any industrial process these need to be carefully tracked and mitigated wherever possible by innovators.   

A note on gases, such as CO2, in fermentation. Fermentation processes often require different gas inputs, for example active growth of aerial mycelium requires CO2 to be added to the growth chamber to inhibit mushrooms from developing. So with any biological process its important to ascertain what gas inputs are needed and what gases are produced/ released at the end. 

Chemistry & Additives

With the production of any material or ingredient it’s essential to understand what, if any, chemicals are used in the process. And to ensure these comply to the relevant Restricted Substances Lists (RSL) and to know if they are also REACH compliant. Proper management, tracking and disposal methods should be in place. 

One of the chemicals or additives of key concern in the field of bioinnovation is Polyurethane, which can be added at multiple stages depending on the technology and process. PU can be used in the material formation, for example in biobased leather alternatives where it is used to bind the biomass and provides key performance attributes. It can also be found in the finish, again adding performance to the end material. 

Another prevalent ‘additive’ we see incorporated into leather alternatives is a textile backer of some kind. Again this addition is included to provide improved performance. A textile can be added either in the growth process as a scaffold or glued on at the end. The addition of this textile will bring it’s own sustainability impacts and so its important to understand and include its environmental impact into the overal biomaterials’. 

Water

At a high level, as with any manufacturing process, understanding water usage and handling is essential. Asking questions such as, how much water is used? Can it be recycled, and if not is wastewater properly treated and processed? 

Specifically with bioinnovations water usage can come into play where crops are grown either as a source of biomass or as a feedstock for an organism. Water is also used in liquid fermentation and tissue culture as a carrier for the cells to grow and multiply. Scaled fermentation facilities have capacities in the 100s of thousands if not millions of liters. Additionally, if the water in fermentation has been used with genetically modified organisms then the correct water treatment procedures should be in place to ensure any traces are removed prior to release back into the environment.

Waste & Co/byproducts

Waste treatment and handling is a consideration with any production process. Any innovator will need to track what waste is generated in their production? And have the appropriate plans in place for its safe and environmental disposal. 

Waste, sometimes also called byproducts, out of processes such as fermentation can include things like biowaste (e.g. cell debris), gases (see emissions section above), and chemicals (e.g. ethanol). With the biowaste it is imperative that this is handled and disposed of correctly, especially if the organism is a GMO.

Another term that may be used in conjunction with fermentation is ‘co-products’, a co-product is a secondary valuable output from a process. In the case of fermentation co-products can be sold for use in a variety of industries such as for ingredients in cosmetics. Thus providing an additional revenue stream and spreading the environmental impact.

End of Use

Whether you use the term End of Life (EoL) or End of Use, what happens to a material or product at the end of its use is determined by multiple factors.

The first aspect that impacts end of use is how the material has been made and what chemistry has been used. For example, if a material is cross linked this will affect its ability to biodegrade. If a material is blended with PU this will likely affect its ability to be recycled. And if a material, like a biosynthetic, is chemically identical to a petrochemical synthetic it will have the same end of life profile and environmental issues (e.g. microfibers) regardless of whether its inputs were biobased. 

The second aspect that affects end of use is how the material is manufactured into a product - can it be disassembled at the end of its useful life and then the component parts reused or recycled? Which leds on to the final large factor affecting end of use - are the correct systems in place in order to get materials back to where they need to be for recycling? An innovator may have developed a material with the ability to exist in an endless closed loop, but if the right waste management systems are not in place to get it back to them then it may well still end up in landfill.

Biobased Technologies

Biobased materials is a broad term, defined in level 1.2 - they can be wholly or partly bio-derived. In order to understanding potential impacts, because ‘biobased’ is such a generic descriptor, it’s important to uncover exactly what is in the material and how it is made. 

The largest group of materials that fall under the ‘biobased’ descriptor are the leather alternatives that combine waste biomass with a binder. The boxes below outline some of the high level questions that should be asked of innovators using biobased technologies. It’s important to note, however, that they may be reluctant to answer some due to IP sensitivities.

1. Biomass input

The majority of biobased technologies use waste biomass as their main ingredient. Understanding where it comes from and its sustainability profile is key.

QUESTIONS TO ASK INNOVATORS:

Feedstock

  • What generation is your feedstock (in this case biomass)? (e.g. if the biomass is not waste from another process, and is grown specifically, could the land be used to grow food or feed?)
  • Are there any potential climate change implications of this feedstock? (e.g. price increases due to drought)
  • What certifications/standards do you have or are looking to achieve in regards to your feedstock?
  • What is the source of the biomass eg: crops, plant waste, agri waste, food waste etc?
  • Is the biomass a GMO or the product of one?
  • Where does the biomass come from (geographic region)?
  • What might be the potential unintended consequences of scaling up production using this biomass?
  • Are pesticides or fertilizers being used to grow these crops?
  • What might be the impact on biodiversity at global scale?
2. Material Formulation/Creation

Biobased materials rely on material science and/ or mechanical formation for their production. Leather alternatives often involve powdered biomass which is mixed with a binder of some kind and backed with a textile. 

QUESTIONS TO ASK INNOVATORS:

Chemistry & Additives

  • What types of chemicals are used in the process? E.g. is the biomass blended with a PU?
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • If yes, what is the plan to move away from these?
Does the material have a textile scaffold and /or backer?
  • If yes, what is its composition and sustainability profile? (e.g. is it recyclable?)
  • If it is glued - does that adhere to RSL lists?
Energy & Emissions
  • What energy source do your processes use?
  • Are you able to track what volumes of emissions are emitted?
Water
  • How much water is used in the process and is it recycled?
  • Is wastewater properly treated and processed?
Waste
  • What waste is generated in production? And how is it disposed of?
3. Material Post Processing
Most biobased alternative leathers do not need tanning in the traditional sense, but may still use some of the associated processes and chemistries.
Questions to ask innovators:
Chemistry & Additives
  • Is the material ‘tanned’ / 'retanned' (or cross-linked)?
  • If yes in what way? e.g as part of the material formulation/creation or once the material is formed?
  • What types of chemicals are used in the process? E.g. chrome
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • If yes, what is the plan to move away from these?
Energy/ Emissions
  • What energy source do your processes use?
  • Are you able to track what volumes of emissions are emitted?
Water
  • How much water is used in the process and is it recycled?
  • Is wastewater properly treated and processed?
Waste
  • What waste is generated in production? And how is it disposed of?
4. Further Chemistry

Most biobased alternative leathers will likely still be ‘finished’ in some way.

QUESTIONS TO ASK INNOVATORS:

Chemistry & Additives

  • What types of chemicals are used in the process? E.g. is the biomass blended with a PU?
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • If yes, what is the plan to move away from these?
Does the material have a textile scaffold and /or backer?
  • If yes, what is its composition and sustainability profile?
  • If it is glued - does that adhere to RSL lists?
Energy & Emissions
  • What energy source do your processes use?
  • Are you able to track what volumes of emissions are emitted?
Water
  • How much water is used in the process and is it recycled?
  • Is wastewater properly treated and processed?
Waste
  • What waste is generated in production? And how is it disposed of?

Stirred Liquid Fermentation Based Technologies

Here we deal with stirred liquid fermentation where the fermentation broth is agitated. This is typically used for the production of bacterial dyes and other bio-derived chemicals, as well as some bacterial cellulose, mycelium materials. 

In most cases, the chemical or biomass produced during fermentation is isolated and undergoes some kind of downstream processing (DSP) before being formulated into the final product. The boxes below outline some of the high level questions that should be asked of innovators using stirred liquid fermentation. It’s important to note, however, that they may be reluctant to answer some due to IP sensitivities.

1. Nutrients / Feedstock

In all fermentation processes organisms need nutrients or ‘feedstock’ in order for them to thrive. 

QUESTIONS TO ASK INNOVATORS:

Feedstock

  • What generation is your feedstock? (e.g. could the land be used to grow food or feed?)
  • What is the principal feedstock being used? (eg: corn, sugarcane, sugarbeet, agri waste, GHGs?)
  • What certifications/standards do you have or are looking to achieve in regards to your feedstock?
  • Is the feedstock a GMO or the product of one?
  • Where does the feedstock come from? (geographic region)
  • What other options are commercially available today?
  • What might be the potential unintended consequences of scaling up the production of this feedstock?
  • Are pesticides or fertilizers being used to grow these crops?
  • Are there any potential climate change implications of this feedstock? (e.g. price increases due to drought)
2. Organism: Bacteria/Yeast/Mycelium
Stirred liquid fermentation can be used with any of the following organism types:
  • Bacteria
  • Yeast
  • Mycelium
Questions to ask innovators:
Organism
  • What type of organism are you using?
  • Is the organism naturally occurring or genetically modified (a GMO)?
  • If yes, what procedures do you have in place in your facilities or those of your partners?

A note on GMOs:
With this type of fermentation, the organism (normally bacteria or yeast) is usually producing an ingredient e.g. a protein, which is separated from the organism via downstream processing. In this instance the ingredient is the product of a GMO, but the final material may not contain any GMO material - the question to ask here is:

  • Is there any GMO material left in your end product?
  • How are you testing for this?

Sometimes with stirred liquid fermentation the organism (usually mycelium) is itself the biomass used to make the material. In this case, if the organism is genetically modified the end product will contain GMO material. 

This is why asking if an innovator’s organism is genetically modified and understanding how their process works, is key. It is then dependent on your stance on GMOs as a brand and in your regulatory geography, as to whether you are able to work with that particular material.

3. Growth Process: Fermentation
Any innovation made using liquid fermentation will have certain resource implications.
Questions to ask innovators:
Chemistry & Additives
  • What other types of ingredients are added in the fermentation process? Do any come from a GMO source?
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • If yes, what is the plan to move away from these?
Energy/ Emissions
  • Is renewable energy being used?
  • Are you able, or working to track what emissions are created during the process?
  • What gasses are used in the process and are any released during or after fermentation?
  • If so, e.g. CO2, how are you tracking that, what is its impact on the final material footprint?
Water
  • How much water is used in the process and is it recycled?
  • Is wastewater properly treated and processed?
Waste
  • What are the outputs of the process and how are you measuring and disposing of them?
  • Are any by/co-products produced during the production process?
  • What are these by/co-products being used for? Or how are they disposed of?
4. Down Stream Processing
In liquid fermentation, there is almost always a need for a downstream processing (DSP) step. This may be a simple drying step or more extensive DSP. 

DSP is essentially a series of steps that extract the desired ingredient from the growth broth to derive a pure and homogeneous product (i.e. a protein).
Questions to ask innovators:
Chemistry & Additives
  • What types of chemicals or additives are used in the process? Do any come from a GMO source?
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • If yes, what is the plan to move away from these?
Energy/ Emissions
  • Is renewable energy being used?
  • Are you able, or working to track what emissions are created during the process?
Water
  • How much water is used in the process and is it recycled?
  • Is wastewater properly treated and processed?
Waste & By/Co-products
  • What are the outputs of the process and how are you measuring and disposing of them?
  • Are any by/coproducts produced during the production process?
  • What are these by/coproducts being used for? Or how are they disposed of?
5. Material Creation & Post Processing
Many leather alternatives do not need tanning in the traditional sense but may still use some of the processes and chemistries.

QUESTIONS TO ASK INNOVATORS:
Chemistry & Additives
  • Is the material ‘tanned’ / 'retanned' (or cross-linked)?
  • If yes in what way? e.g as part of the liquid formulation or once the material is formed?
  • What types of chemicals are used in the process? E.g. chrome
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • If yes, what is the plan to move away from these?
  • Does the material have a textile scaffold and/ or backer?
  • If yes, what is its composition and sustainability profile? (e.g. is it recyclable?)
  • If it is glued - does that adhere to RSL lists?
Energy & Emissions
  • What energy source do your processes use?
  • Are you able to track what volumes of emissions are emitted?
Water
  • How much water is used in the process and is it recycled?
  • Is wastewater properly treated and processed?
Waste
  • What waste is generated in production? And how is it disposed of?
6. Further Chemistry
Most leather alternatives will likely still be ‘finished’ in some way.
Questions to ask innovators:
Chemistry & Additives
  • Is the material finished? 
  • If yes in what way? e.g. as part of the production (step 5) i.e. reverse lay-up or at the end?
  • What types of chemicals are used in the process? E.g. PU finishes, cross linkers etc.
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • If yes, what is the plan to move away from these?
Energy & Emissions
  • What energy source do your processes use?
  • Are you able to track what volumes of emissions are emitted?
Water
  • How much water is used in the process and is it recycled?
  • Is wastewater properly treated and processed?
Waste
  • What waste is generated in production? And how is it disposed of?
  • How are waste gasses from any finishing processes treated?

Static (Liquid or Solid) Fermentation Based Technologies

Here we deal with static liquid OR solid fermentation. In either case it usually takes the form of some kind of tray system. This is typically used for the production of bacterial cellulose or mycelium materials. 

Materials coming out of this process will be grown into the final sheet form, though they will undergo further chemical and/or mechanical treatments. The post-processing will include rendering the living organism inert.The boxes below outline some of the high level questions that should be asked of innovators using static fermentation. It’s important to note, however, that they may be reluctant to answer some due to IP sensitivities

1. Nutrients / Feedstock


In all fermentation processes organisms need nutrients or ‘feedstock’ in order for them to thrive.

QUESTIONS TO ASK INNOVATORS:
Feedstock
  • What generation is your feedstock? (e.g. could the land be used to grow food or feed?)
  • What feedstock is being used? (eg: corn, sugarcane, sugarbeet, agri waste, GHGs)
  • What feedstock is being used? (eg: corn, sugarcane, sugarbeet, agri waste, GHGs)
  • What certifications/standards do you have or are looking to achieve in regards to your feedstock?
  • Where does the feedstock come from (geographic region)?
  • What other options are commercially available today?
  • What might be the potential unintended consequences of scaling up the production of this feedstock?
  • Are pesticides or fertilizers being used to grow these crops?
  • Is the feedstock a GMO or the product of one?
  • Are there any potential climate change implications of this feedstock? (e.g. price increases due to drought)
2. Organism: Bacteria/Yeast/Mycelium

Static fermentation is usually carried out with the following organism types:

  • Bacteria
  • Mycelium
Questions to ask innovators:
Organism
  • What type of organism are you using?
  • Is the organism naturally occurring or genetically modified (a GMO)?
  • If yes, what procedures do you have in place in your facilities or those of your partners?

A note on GMOs:
In this type of fermentation the organism either is the final material (i.e. mycelium) or is trapped within the final material (i.e. bacterial cellulose). 

Therefore, if the organism being used is genetically modified then the end product will contain genetically modified material. 

This is why asking if an innovator’s organism is genetically modified and understanding how their process works, is key. It is then dependent on your stance on GMOs as a brand and in your regulatory geography, as to whether you are able to work with that particular material.

3. Growth Process: Fermentation
Any innovation made using static (liquid or solid) fermentation processes will have certain resource implications. With this type of fermentation the organism is almost always bioassembling the material itself, so there is no need for a subsequent material creation stage.
Questions to ask innovators:
Chemistry & Additives
  • What other types of ingredients are added in the process? Do any come from a GMO source?
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • Does the material have a textile scaffold that is added during the growth process?
  • If yes, what is its composition and sustainability profile? (e.g. is it recyclable?)
Energy & Emissions
  • Is renewable energy being used?
  • Are you able, or working to track what emissions are created during the process?
  • What gasses are used in the process and are any released during or after fermentation?
  • If mycelium is being used, ask if the growth process is passive or active (active growth involves adding CO2 into the growth chamber to inhibit mushroom growth) 
Water
  • How much water is used in the process and is it recycled?
  • What water treatment procedures are in place?
Waste & By/Co-products
  • What are the outputs of the process and how are you measuring and disposing of them?
  • Are any by/co-products produced during the production process?
  • What are these by/co-products being used for? Or how are they disposed of?
4. Material Post Processing
Many leather alternatives do not need tanning in the traditional sense but may still use some of the processes and chemistries.
Questions to ask innovators:
Chemistry & Additives
  • Is the material ‘tanned’ / 'retanned' (or cross-linked)?
  • What types of chemicals are used in the process? E.g. chrome
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • If yes, what is the plan to move away from these?
  • Does the material have a textile backer?
  • If yes, what is its composition and sustainability profile? (e.g. is it recyclable?)
  • If it is glued - does that adhere to RSL lists?
Energy & Emissions
  • What energy source do your processes use?
  • Are you able to track what volumes of emissions are emitted?
Water
  • How much water is used in the process and is it recycled?
  • Is wastewater properly treated and processed?
Waste
  • What waste is generated in production? And how is it disposed of?
5. Further Chemistry
Most leather alternatives will likely still be ‘finished’ in some way.
Questions to ask innovators:
Chemistry & Additives
  • Is the material finished? 
  • If yes in what way? 
  • What types of chemicals are used in the process? E.g. PU finishes, cross linkers etc.
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • If yes, what is the plan to move away from these?
Energy & Emissions
  • What energy source do your processes use?
  • Are you able to track what volumes of emissions are emitted?
Water
  • How much water is used in the process and is it recycled?
  • Is wastewater properly treated and processed?
Waste
  • What waste is generated in production? And how is it disposed of?
  • How are waste gasses from any finishing processes treated?

Tissue Engineering Based Technologies

Here we deal with materials produced from mammalian (animal) cells. Various methods may be used, from using a stirred bioreactor through to more static forms of cell culture.  

If the materials coming out of this process are grown in sheet form, they may be grown on some kind of substrate, for example a textile ‘scaffold’. These materials will likely be comprised of some form of collagen protein and will therefore need to be treated in a similar way to leather to prevent putrefaction. The boxes below outline some of the high level questions that should be asked of innovators using static fermentation. It’s important to note, however, that they may be reluctant to answer some due to IP sensitivities.

1. Nutrients / Feedstock

As a result, innovators are moving away from its use, but serum free media (liquid used to grow cells in) is not without its challenges. The ingredients that are found in FBS (such as proteins, carbohydrates, growth factors, cytokines, fats, vitamins, minerals, and hormones) need to be replaced via other sources.

Cells need nutrients or a ‘feedstock’ in order to thrive. In the case of mammalian cells, they have traditionally needed an ingredient called FBS (fetal bovine serum) which is derived from the blood of deceased calf fetuses. This is both ethically problematic, as well as being expensive as an ingredient and not available at scale.

Questions to ask innovators:
Feedstock
  • Is FBS being used?
  • If yes, is there a plan to transition to serum free and what is the timeline for that?
  • What other types of ingredients are used in the cell media (i.e. feedstocks)?
  • What generation is your feedstock? (e.g. could the land be used to grow food or feed?)
  • What certifications/standards do you have or are looking to achieve in regards to your feedstock?
    Where do the ingredients come from (geographic region)?
  • What other options are commercially available today?
  • What might be the potential unintended consequences of scaling up the production of this media?
  • Are any of the ingredients a GMO or the product of one?
  • Are there any potential climate change implications of this feedstock? (e.g. price increases due to drought)
2. Organism: Mammalian Cells

Tissue engineering for the creation of leather-like materials uses mammalian (animal) cells. Although several companies now use this approach to grow consumer materials, e.g. Vitrolabs, it is still highly novel and significantly more expensive than other processes.

Questions to ask innovators:
Organism
  • What cell type are you using? 
  • What animal do they come from?
  • Are the cells from a biopsy or a cell bank?
  • Are the cells genetically modified in any way?
  • If yes, what procedures do you have in place in your facilities or those of your partners?
3. Growth Process:Tissue Culture
Tissue engineering, when compared to other biomaterial processes, may have additional resource implications. Single use plastic, energy and water intensive steps such as autoclaving may be required due to a need for strict sterility. 

Depending on the method deployed, the cell may be bioassembling the material itself, in which case there is no need for a subsequent material creation stage
Questions to ask innovators:
Chemistry & Additives
  • What other types of ingredients are added in the process? Do any come from a GMO source?
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • Does the material have a textile scaffold that is added during the growth process?
  • If yes, what is its composition and sustainability profile? (e.g. is it recyclable?)
Energy & Emissions
  • Is renewable energy being used?
  • Are you able, or working to track what emissions are created during the process?
  • What gasses are used in the process (e.g. in incubators) and are any released during or after?
Water
  • How much water is used in the process and is it recycled?
  • What water treatment procedures are in place?
Waste & By/Co-products
  • How many consumables are used in the growth process? E.g. plastic flasks 
  • Are plastics recycled or are you using reusable/ re-sterilizable vessels instead? 
  • What are the outputs of the process and how are you measuring and disposing of them?
  • Are any by/co-products produced during the production process?
  • What are these by/co-products being used for? Or how are they disposed of?
4. Material Post Processing
Of all the biomaterial alternatives, tissue engineered ‘leathers’ are most likely to fit seamlessly into tanning infrastructure. They should largely be able to use existing chemistries due to the fact they are built from the same building blocks as leather i.e. collagen. However, the purer, more delicate, structure may still require significant alterations to chemical formulations, concentrations and processes.
Questions to ask innovators:
Chemistry & Additives
  • Is the material ‘tanned’ / 'retanned' (or cross-linked)?
  • What types of chemicals are used in the process? E.g. chrome
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • If yes, what is the plan to move away from these?
  • Does the material have a textile backer?
  • If yes, what is its composition and sustainability profile?
  • If it is glued - does that adhere to RSL lists?
Energy & Emissions
  • What energy source do your processes use?
  • Are you able to track what volumes of emissions are emitted?
Water
  • How much water is used in the process and is it recycled?
  • Is wastewater properly treated and processed?
Waste
  • What waste is generated in production? And how is it disposed of?
5. Further Chemistry
Representing the closest approximation to leather, tissue engineered collagen materials will likely still need to be ‘finished’.
Questions to ask innovators:
Chemistry & Additives
  • Is the material finished? 
  • What types of chemicals are used in the process? E.g. PU finishes, cross linkers etc.
  • How are chemicals recovered, recycled or disposed of?
  • Are you REACH compliant and are any of the chemicals used on Restricted Substances Lists e.g. ZDHC’s?
  • If yes, what is the plan to move away from these?
Energy & Emissions
  • What energy source do your processes use?
  • Are you able to track what volumes of emissions are emitted?
Water
  • How much water is used in the process and is it recycled?
  • Is wastewater properly treated and processed?
Waste
  • What waste is generated in production? And how is it disposed of?
  • How are waste gasses from any finishing processes treated?

GMOs

The term ‘genetically modified organism’ (GMO) is used to describe ‘a plant, animal, or microorganism that has had its genetic material (DNA) altered through a process called genetic engineering’. Around 90% of agricultural crops like corn, soy and cotton are genetically modified in the USA. In the USA, GMOs are regulated by three federal agencies: The U.S. Food and Drug Administration (FDA), U.S. Environmental Protection Agency (EPA), and U.S. Department of Agriculture (USDA).

GMOs may be used at various stages in the production of a biomaterial. Commonly a genetically modified plant may be used as a feedstock for a fermentation process, for example, sugar obtained from corn. In fermentation, a microorganism such as a bacterium or yeast cell might be genetically engineered to produce an ingredient, for example to produce a dye such as by Colorifix. Bacterial and fungal strains may also be genetically modified to produce materials by bioassembly. Here a GMO organism grows a material structure and is embedded within the final material, even if rendered inert, for example a bacterial cellulose or mycelium material. Finally, materials may come into contact with genetically modified enzymes for processing or finishing treatments.

GMOs are used globally in industrial biotech to manufacture ingredients for food (e.g. vanillin), pharma (e.g. penicillin), personal care (e.g. perfume scents such as vetiver) along with many others. No work has yet been done specifically related to understanding GMOs in the context of the latest consumer biomaterial innovations. It is likely that regulations are behind the scientific advances in the field. It should be noted that regulation varies tremendously according to geography, with the EU being the most tightly regulated.

Some questions to ask when assessing risk around GMOs include:

  • Are GMOs used in any part of this process, if so where and how?
  • What is the protocol for the safe use and disposal of that organism?
  • What are the tests to determine presence of genetically modified matter in a final product?
  • Subjective and unmeasurable claims
  • What regulations govern the use of GMOs in this context?
  • What are the correct procedures when shipping / receiving materials containing GMOs (even if rendered inert)?

Tanning

Tanning, cross linking, or other chemical treatments and finishes are usually needed for most leather alternatives to level up in terms of durability, performance or aesthetic requirements.

However, there are no blanket ‘tanning’ solutions for leather alternatives, the majority of which are not made of collagen. Mycelium is primarily composed of chitin proteins, while bacterial cellulose is a carbohydrate not a protein. So, unsurprisingly, we don’t yet have optimized chemistries for these novel biomaterials.

There is an important trade off here. In order to achieve similar performance or durability to leather, innovators may be taking a material which is wholly, or almost, 100% biobased, and adding non-biobased chemicals or polymers, such as PU either as a binder or as part of a finish. This has two effects. Firstly if the chemical is not 100% biobased it will mean the material itself can no longer be classed as 100% biobased. Secondly, any kind of cross-linking of material structure is oppositional to biodegradation. This is true of leather too. However it is still possible to recycle cross linked materials.

Cross-linking aims to increase material stability over time delivering long-lasting durability. This can be at odds when a brand wants a high-performing, 100% biobased material that will naturally compost. Mirum™ is one of the very few options here, which may not be the desired aesthetic.

With biomaterials, in the equation of durability, performance, aesthetic and end of life, some form of compromise is inevitable.

PU & Textiles

With very few exceptions, the new generation of biomaterials seeking to offer an alternative to leather lean on PU and textile substrates to meet customers' performance specifications. The vast majority of leather alternatives are created using some form of waste biomass which is either macerated (retaining some fibers) or, more commonly, ground into a powder. This is then mixed into a liquid suspension which can coat a textile. Most innovations rely on existing coated textile roll-to-roll production lines.

The reality is that the performance of these biomaterials has more to do with the polymer binder and textile backer than the powdered biomass. Binders vary, some use natural rubber, a few use biopolyols, but the vast majority turn to polyurethane (PU). PU can be either fossil-derived (petrochemical) or biobased. Most biobased PU formulations still contain a % of virgin petrochemicals. Confusingly, some 100% fossil based PUs degrade more readily than biobased versions, so biocontent % is not an indicator of greater degradability at end of life. If not used as a binder, many finishing chemistries will also include polyurethane for durability. Many leathers also feature PU in their top coats.

Textile backing materials can of course be woven, knitted or non-woven and fossil-derived such as a knitted polyester or from natural fibers such as knitted organic cotton. Innovators generally look to brand guidance on their preference for textile backer. The textile is counted in the total % of the material’s biocontent for an LCA, and will also affect price.

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